An in vitro micropropagation protocol has been standardized via direct and indirect methods from Shoot tip, Nodal and leaf explants of Diplocyclos palmatus belongs to family Cucurbitaceae is an endemic medicinal plant used for the treatment of reproductive medicine Female infertility, aphrodisiac and leucorrhoea. Murashige and Skoog, (1962) medium along with the various hormone compositions were used. 70% alcohol for few seconds and rinsed three times with distilled water. The explants were brought to the inoculation chamber and surface sterilized with 0.1% HgCl2 for 3-5 min and again washed with sterile distilled water for 5-7 times. MS medium supplemented with various concentrations of NAA, Kin, AgNPs and BAP. The multiple shoots were induced from nodal explants the MS medium fortified with NAA (1.5mg/L), Kin (1.0mg/L), AgNPs (1.0mg/L) and BAP (3.0mg/L). The shoots were induced from shoot tip explants the MS medium supplemented with NAA (1.0mg/L), Kin (0.5mg/L), AgNPs (1.0mg/L) and BAP (2.5mg/L).Then shoots were transferred to rooting medium supplemented with NAA (2.0mg/L) IAA (1.5mg/L), AgNPs (2.0mg/L) and IBA (2.5mg/L). Acclimatization was carried out on artificial soil survival rate. Callus culture was initiated and established from leaf explants MS medium supplemented with various concentrations of NAA, BAP and 2, 4-D. The leaf callus was formed in NAA (1.5mg/L), BAP (2.0mg/L) and 2, 4-D (2.0mg/L) Greenish and Yellowish white compact callus was induced.
Rethinam R and Jeyachandran R, 2016. In vitro micropropagation of diplocyclos palmatus (l.) c. jeffrey- an endemic medicinal plant. International Journal of Current Science and Technology, Vol.4, Issue, 9, pp. 271-274.