Orthogonal test design to optimize the production of 2,3-butanediol on fermentation medium by klebsiella pneumonia zh-1

Author: 
Li Li., Jianguo Xu and Qingping Hu

There has been a growing interest in the fermentative production of 2,3-butanediol (2,3-BD) because of its wide applications in the chemical, food, pharmaceutical, and fuel industries. In this study, the increase in 2,3-BD production was achieved by optimizing the fermentation medium. The strains of Klebsiella pneumonia ZH-1, was studied by single factor and orthogonal test to select the best fermentation medium. Carbon source, nitrogen source, mineral salt and metal ion have an important effect on the cell growth of K. pneumonia ZH-1 and the production of 2,3-BD. The optimal fermentation medium were determined as follows (g/L): glucose 60; yeast extract 10; KH2PO4 4; K2HPO4 24; (NH4)2SO4 2; citrate sodium 1; CuSO4 0.04; EDTA 0.05. Among them, the biggest influence is yeast extract on this test result. Under the optimal conditions, the output of 2,3-BD can reach 13.5 g/L, which provide theoretical basis for industrialized production of 2,3-BD and the instruction.

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Life Science
Volume: 
Volume5
Issue: 
8
Year of Publication: 
2017
How to Cite this article: 

Li Li., Jianguo Xu and Qingping Hu, 2017.,Orthogonal test design to optimize the production of 2,3-butanediol on fermentation medium by klebsiella pneumonia zh-1, International Journal of Current Science and Technology, Vol.5, Issue, 8, pp. 484-488