The development and use of an improved cellulolytic bacterial strain, through chemical mutagenesis, for the efficient bioconversion of MSW into value-added compost was studied under in vitro condition. In the present study, the degrading soil (MSW) samples were collected from the dumping site of Chidambaram municipality. Five cultures of cellulolytic bacteria screened and designated as “AU” series and numbered randomly. The production of cellulase enzyme by representative isolates was assayed under in vitro condition and revealed that the isolate AU-3 recorded a maximum production of cellulases enzyme when compared to other isolates. The phenotypic characterisation of the efficient isolate viz., AU-3 revealed that the organism belonged to the genera Bacillus and species subtilis. A putative mutant AU-3-M screened from wild AU-3 after mutagenesis with N-methyl N-nitro N-nitrosoguanidine (NTG), as a chemical mutagenic agent. It was observed that the mutant AU-3-M produced a higher amount of cellulases when compared to the wild type AU-3 strain. Moreover, the MSW inoculated with the mutant strain, AU-3-M recorded the highest weight loss on 60th day of observation than the wild strain under in vitro condition. It is concluded that the strain improvement of cellulolytic bacteria by mutation has high biotechnological potential for the bioconversion of MSW into value-added product viz., compost.
Lalith Varadhan S and Mohan S. 2017,International Journal of Current Science and Technology, Development and use of an improved cellulolytic strain for the efficient bioconversion of municipal solid waste (msw) into value-added compost, Vol.5, Issue, 3, pp.357-360