Cryopreservation of genetic material collected post-mortem from male gray brocket deer mazama gouazoubira fischer, 1814

Author: 
Assumpção, Teresinha Inês and Santos, André Luiz Quagliatto

The objective of this study was to collect and cryopreserve spermatozoa collected post-mortem from the tail of the epididymis of a gray brocket deer (Mazama gouazoubira) in order to preserve the genetic material of the species. Spermatic cells of an adult animal were collected 5 hours after it was run over. The tails of the epididymides were dissected and sectioned, pressed to eliminate the cells, and washed in Dulbecco PBS. Physical and morphological analyses of the semen were based on routine methods. Semen was diluted 1:1 in Botubov® dilution medium in a final concentration of 810 million cells/mL. Diluted semen was cooled to 5°C and frozen at -196°C in 0.25 mL straws following a slow freezing curve. Thawing of the straws was carried out at 37°C/ 30 s. Total volume of semen was 3 mL, initial motility was 80%, vigor 3, and sperm cell concentration, 1.62 x 109 cells/mL, with 43% abnormal spermatozoa. After thawing, motility was 30% and vigor 3. It was concluded that gray brocket deer (Mazama gouazoubira) spermatozoa collected from the epididymis several hours after the death of the animal may be cryopreserved with good viability and used in in vitro assays.

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Teaching and Research Laboratory
Volume: 
Volume5
Issue: 
9
Year of Publication: 
2017
How to Cite this article: 

Assumpção, Teresinha Inês and Santos, André Luiz Quagliatto, 2017, Cryopreservation of genetic material collected post-mortem from male gray brocket deer mazama gouazoubira fischer, 1814, International Journal of Current Science and Technology, Vol.5, Issue, 9, pp. 510-512