Assisted reproduction in alligators: physical and morphological characterization of spectacled caiman semen caiman crocodilus linnaeus, 1758

Author: 
Assumpção, Teresinha Inês, Santos, André Luiz Quagliatto, Santos, Raísa Brito and Macedo, Gustavo Guerino

The objective of this study was to understand the physical and morphological characteristics of spectacled caiman semen (Caiman crocodilus). Twenty-five free-living caimans from the state of Mato Grosso, Brazil (Amazon region) were used. Semen was collected by electro ejaculation under physical restraint. For the physical analysis of sperm cell motility and vigor, a drop of semen was placed on a glass slide and covered with a coverslip for optical microscopy. Semen concentration was determined in a Neubauer chamber and sperm morphology was determined by wet-mount preparation for phase-contrast microscopy. The volume of semen ranged from 200 to 500 µL; it was milky white; motility ranged from 80 to 90%; and mean vigor was from 2 to 4. Mean spermatozoon concentration in semen was 2.14 ± 1.98 billion/ml. As for sperm cell morphology, they were filiform with a pointy end and s-shaped heads; total length was 80.98 ± 1.29 µm. Percentage of sperm abnormalities was 14.75 ± 1.55%. Spectacled caiman semen showed optimum physical and morphological characteristics, with adequate quantity and quality, indicating high fertility rates. There are no definitions of ideal reproductive parameters for the species, and further studies are necessary on these reptiles.

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Wild Animals
Volume: 
Volume5
Issue: 
10
Year of Publication: 
2017
How to Cite this article: 

Assumpção, Teresinha Inês et al.2017, Assisted reproduction in alligators: physical and morphological characterization of spectacled caiman semen caiman crocodilus linnaeus, 1758, International Journal of Current Science and Technology, Vol.5, Issue, 10, pp. 513-516